Suzaki is situated in a jutting lumpy shore, with a wide swash zone (34°39′N, 138°57′E). clavigera, goose barnacle Capitulum mitella (Linnaeus, 1758), and the Mytilidae bivalves Septifer virgatus (Wiegmann, 1837). clavigera individuals and 60 dead individuals of each prey were collected in June to August 2013.
Shirahama is a relatively isolated rocky shore surrounded by a sandy beach (34°41′N, 138°58′E). We used another Mytilidae species Arcuatula senhousia (Benson in Cantor, 1842) because S. To overcome the effect of ontogenetic change in prey preference, we collected appropriately similar-sized individuals of each species: the adult predatory R. All the species were collected and investigated before the spawning season. Before the experiment, the predator was placed in flowing seawater for 1 month, under starvation conditions, and the prey species were stored at −60°C.
Taken together, our findings suggested the prey choice test is the most appropriate method of identifying the prey preferences of muricid snails when large numbers of live preys are difficult to collect.
Predation is widely considered an important process that regulates the spatial distribution and population dynamics of animals, which in turn determines the community structure of various ecosystems.
We propose an appropriate method for investigating the prey preferences of the muricid snail Reishia clavigera (Küster, 1860) with limited collection of live prey.
We compared 3 methods for examining the prey preference.
The muricid snail Reishia clavigera (Küster, 1860) is a carnivorous predator in the intertidal rocky shores throughout temperate and tropical regions of the Western Pacific, including Japan.
Species belonging to Muricidae have varied diets (including barnacles, limpets, and bivalves) with each species having specific prey preferences .
The last method was a stable isotope analysis of R. Using live prey, bivalves were the most preferred prey, but goose barnacle was the most preferred prey species in experiments using dead prey.
The first was a predation experiment, conducted with dead prey instead of live prey.